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DNA Technology

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1. Describe DNA sequencing with reference to the cloning of a DNA fragment in a bacteriophage M13 based cloning vector.

2. Describe the use of a expression vector to isolate the gene from a cDNA library that codes for a specific protein.

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Solution Summary

Short paragraphs with references for each question that examines DNA technologies of sequencing and gene siolation.

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1. The Sanger method of sequencing involves the random premature termination of base pair extension using dideoxy-bases. For more information on sequencing see http://en.wikipedia.org/wiki/DNA_sequencing. In the case of a sequence cloned into the M13 based vector the primer used to start the extension reaction would be the M13 forward or reverse primer. By using each of these in separate sequencing reaction the forward ...

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