1. The Sanger method of sequencing involves the random premature termination of base pair extension using dideoxy-bases. For more information on sequencing see http://en.wikipedia.org/wiki/DNA_sequencing. In the case of a sequence cloned into the M13 based vector the primer used to start the extension reaction would be the M13 forward or reverse primer. By using each of these in separate sequencing reaction the forward ...
Short paragraphs with references for each question that examines DNA technologies of sequencing and gene siolation.