How many total hydrogen bonds would exist between the ff. strands of DNA and the complementary strands? a.) GCATGC b.) TATGGC A strand of DNA has the base sequence GCTTAG. Write the base sequence for the complementary strand. Remember, the DNA and RNA strands are written from the 5' to the 3' end
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What is the function of "stress" proteins under heat shock conditions and how is this related to their function under normal conditions? I explain the mechanism underlying their actions in the cell.
Are all transcribed genes translated into proteins and why?
Which statement is incorrect about macromolecules? a. Polypeptides are composed of amino acids linked by amide bonds. b. RNA is composed of ribonucleotides linked by phospho ester bonds. c. Carbohydrates polymers are composed of monosaccharides linked by ester bonds. d. RNA is composed of four different monomers. Which is
You are studying the initiation of chromosomal DNA replication in a novel bacterium that grows at 75°C. To this end you have isolated temperature sensitive replication mutants in twelve different proteins/genes derived from this strain. You call these mutations hot1-hot12. Your hope is to use these mutations to identify protein
1. Find a model of a human homeodomain/DNA complex. a. How many models do you find? b. What method of structure determination produced the first of these models? c. View the first model on the list with QuickPDB or your favorite molecular viewer. What secondary structural element(s) (helix, sheet, coil) interact with DNA? d
The galactose represser protein from E. coli has a pI of about 5.9. While purification protocols were being designed, it was found to bind to a Mono-S column at pH values of 7 and below. (Mono-S columns have S-type sulfonic acid groups attached to the resin and are strong cation exchangers.) What is unusual about this observ
A. Explain the experimental results that are seen in the absence of microsomes (Figures 8-10, lanes 1 to 4). B. Using the three criteria outlined in the problem decide whether the experimental results in the presence of microsomes (lanes 5 to 8) indicate that the protein is translocated across microsomal membranes. Explain the
How does transcription differ from DNA replication? How is it the same? What is the role of complementary base-pairing in each? If a section of the DNA strand to be transcribed is CTAATGT, what is the corresponding section of the mRNA? *PLEASE SIMPLIFY*
What background material did Watson and Crick have available for developing a model of DNA? What was their contribution to the building of the model?
How do amino acid sequences function as a "biological clock?" This process is described.