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Translocation of proteins

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A. Explain the experimental results that are seen in the absence of microsomes (Figures 8-10, lanes 1 to 4).
B. Using the three criteria outlined in the problem decide whether the experimental results in the presence of microsomes (lanes 5 to 8) indicate that the protein is translocated across microsomal membranes. Explain the migration of the proteins in lanes 5, 6 and 8.
C. Is the protein anchored in the membrane, or is it translocated all the way through the membrane?

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Solution Summary

Questions are presented about the translocation of proteins.

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No microsomes: result ---> protein synthesized
No microsomes, protease added: result ---> protein degraded
No microsomes, protease added, detergent added: result ---> protein degraded (no surprise here!)
No microsomes, endoH added: result ---> no change in size of protein

Summary: these are controls showing that the protein can be synthesized in the absence of the microsome preparation. The controls ...

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