Translocation of proteins
Not what you're looking for?
A. Explain the experimental results that are seen in the absence of microsomes (Figures 8-10, lanes 1 to 4).
B. Using the three criteria outlined in the problem decide whether the experimental results in the presence of microsomes (lanes 5 to 8) indicate that the protein is translocated across microsomal membranes. Explain the migration of the proteins in lanes 5, 6 and 8.
C. Is the protein anchored in the membrane, or is it translocated all the way through the membrane?
These questions are posed.
Purchase this Solution
Solution Summary
Questions are presented about the translocation of proteins.
Solution Preview
A
No microsomes: result ---> protein synthesized
No microsomes, protease added: result ---> protein degraded
No microsomes, protease added, detergent added: result ---> protein degraded (no surprise here!)
No microsomes, endoH added: result ---> no change in size of protein
Summary: these are controls showing that the protein can be synthesized in the absence of the microsome preparation. The controls ...
Purchase this Solution
Free BrainMass Quizzes
Labor Comfort Measures
Do you know how to comfort someone in labor?
Basic Concepts in Neuroscience
This quiz provides a review of the basic concepts in neuroscience.
How Well Do You Know Your Body?
This quiz will assess the different systems of the human body. It will examine everything from the organs to the cellular processes that occur.
Bacterial Genetics
This quiz test your knowledge of the genetics of bacteria.
Parts of the Brain
This quiz will test your knowledge on different areas of the brain.