Purchase Solution

Translocation of proteins

Not what you're looking for?

Ask Custom Question

A. Explain the experimental results that are seen in the absence of microsomes (Figures 8-10, lanes 1 to 4).
B. Using the three criteria outlined in the problem decide whether the experimental results in the presence of microsomes (lanes 5 to 8) indicate that the protein is translocated across microsomal membranes. Explain the migration of the proteins in lanes 5, 6 and 8.
C. Is the protein anchored in the membrane, or is it translocated all the way through the membrane?

These questions are posed.

Purchase this Solution
Solution Summary

Questions are presented about the translocation of proteins.

Solution Preview

A
No microsomes: result ---> protein synthesized
No microsomes, protease added: result ---> protein degraded
No microsomes, protease added, detergent added: result ---> protein degraded (no surprise here!)
No microsomes, endoH added: result ---> no change in size of protein

Summary: these are controls showing that the protein can be synthesized in the absence of the microsome preparation. The controls ...

Purchase this Solution
Free BrainMass Quizzes
The Heart

This quiz test the understanding of the heart and some of its parts. It is important to understand how the heart functions and what makes it function.

Hemophilia: Fact or Fiction

Do you know the truth about hemophilia? Test your knowledge here.

Pregnancy Knowledge

How much do you know about being pregnant? Test your Pregnancy IQ with this quiz!

Human Anatomy- Reproductive System

Do you know your reproductive anatomy?

Comfort Measures For Labor

Are you ready to doula someone through labor?

View More Free Quizzes
Related BrainMass Solutions

  • Multiple choice questions in Cell Biology

    import folded, even oligomeric, proteins, which distinguishes the peroxisomal translocation machinery from the well-characterized translocons of other organelles. 10.

  • Protein Targetting and the lac Operon

    The proprotein is transferred to protein complex known as the Translocase of Outer Membrane (TOM) of the outer membrane. Translocation is an ATP-dependent process mediated by TOM that transfers the linear proteins to the intermembrane space.

  • Smad transcription factors

    dephosphorylation of SMAD cause them to return to the cytoplasm for another round of receptor mediated phosphorylation and nuclear translocation. (1) The nuclear import and export of SMADs proteins is well studied in Smads 2, 3, and 4.

  • Translation in Prokaryotes and Eukaryotes

    The translocation of proteins across membranes involves the signal sequence, that is, a short amino acid sequence at one end that functions like a postal or zip code for the target organelle.

  • Nuclear Transport Mechanisms

    Gene regulatory signals are transduced into the nucleus through the import of specific regulatory proteins. Many of these nuclear proteins contain a "classical" nuclear localization signal (NLS).

  • Antigenic processing

    Then the TAP transporter will be responsible for the translocation of the cytosolic peptides to the ER where the MHCI complex is found.

  • DNA translocation and cancer

    135013 How would DNA translocation produce cancer? How would DNA translocation produce cancer? The most notable example of a DNA translocation occurs in the Philadelphia translocation that leads to Chronic Myelogenous Leukemia (CML).

  • DNA Replication and Bacterial Infections

    Erythromycin interferes with aminoacyl translocation, preventing the transfer of the tRNA bound at the A site of the rRNA complex to the P site of the rRNA complex.

  • Encoding a protein with a pair of conflicting signal sequences

    73582 Encoding a protein with a pair of conflicting signal sequences Imagine you have engineered a set of genes, each encoding a protein with a pair of conflicting signal sequences that specify different compartments.

View More Related Solutions