Purchase Solution

The Order of Elution in Column Chromatography

Not what you're looking for?

Ask Custom Question

In normal phase column chromatography, which solvent has more eluting power: petroleum ether or dicloromethane? In what way is the eluting power of a solvent related to its polarity? In normal phase column chromatography, how does the principle of like dissolve like explain the affinity of a compound for the mobile phase, relative to the stationary phase.

A mixture containing compounds 9,10,11 was separated by normal phase column chromatography, using neutral alumina as the stationary phase, and diethyl ether as the mobile phase. Label each compound according to its relative polarity (nonpolar, slightly polar, moderately polar, and/or very polar). Predict the order (1st,2nd, 3rd) in which the compounds 9,10 and 11 will elute from the column. Diagrams of these compounds are attached.

Attachments
Purchase this Solution
Solution Summary

The order of elution is discussed for column chromatograms.

Solution Preview

As the solvent polarity increases, more solutes will dissolve in it. Hence, eluting power will also increase. Dichloromethane is more polar than petroleum ether and will thus ...

Purchase this Solution
Free BrainMass Quizzes
Organic Chemistry Naming: Alkanes

This is a quiz which is designed to assist students with learning the nomenclature used to identify organic compounds. This quiz focuses on the organic compounds called Alkanes.

Thermochemistry

The quiz helps in revising basic concepts about thermochemistry.

Periodic table

Match Elements with their Symbols

Elements are provided: choose the matching one- or two-letter symbol for each element.

General Chemistry - Classification of Matter

This test will assess your knowledge on the classification of matter which includes elements, compounds and mixtures.

View More Free Quizzes
Related BrainMass Solutions

  • Basis for metal affinity chromatography

    392974 Chromatography Consider immobilized metal affinity chromatography, such as a Ni2+ column. For this type of chromatography, describe the basis of separation of the protein components.

  • Enantiomers and Order of Elution

    This solution describes in 303 words how to find the order of enantiomers through transformation and the four steps of the chiral recognition mechanism.

  • High-performance liquid chromatography

    Reverse Phase HPLC: The stationary phase is modified in order to make it non-polar (usually by appending long hydrocarbon chains) and the order of elution is opposite to that for normal phase HPLC. This is the most common type of HPLC.

  • Affinity Chromatography

    The elution contains less albumin than the total amount in the bulk serum sample because a large portion of the protein did not bind to the column and was lost in the flow through.

  • Ethyl Acetate as an Elution Solvent

    As the above information, the impure caffeine mixture can be separated only in a soluble solvent as an elution and we can get some separated spots in the TLC plate that we can purify each spot individually by using Column Chromatography.

  • Why Didn't my Chromatography Experiment Work Properly?

    What you witnessed was a "tailed" elution. In other words, because the solvent was not quite polar enough, it spread the fraction across too much of the length of the column.

  • Chromatography Elution

    34410 Chromatography Elution Question: Sketch the expected chromatographic elution profile (protein concentration vs elution volume) if a mixture of these proteins is applied to a weak anion exchange resin such as DEAE cellulose at a pH of 7.

  • PI (isoelectric poin)

    In general, the steps involved in column chromatography with an ion-exchange resin are as follows: (see http://matcmadison.edu/biotech/resources/proteins/labManual/chapter_4/section4_4.htm for a great overview of this process). 1. set up the column

  • Binding and Elution of Peroxidase

    In order to elute the peroxidase, you need a buffer at the isoelectric point of 9.10, where peroxidase has an overall neutral charge and therefore will no longer bind to the column resin and will wash out of the column.

View More Related Solutions