In normal phase column chromatography, which solvent has more eluting power: petroleum ether or dicloromethane? In what way is the eluting power of a solvent related to its polarity? In normal phase column chromatography, how does the principle of like dissolve like explain the affinity of a compound for the mobile phase, relative to the stationary phase.
A mixture containing compounds 9,10,11 was separated by normal phase column chromatography, using neutral alumina as the stationary phase, and diethyl ether as the mobile phase. Label each compound according to its relative polarity (nonpolar, slightly polar, moderately polar, and/or very polar). Predict the order (1st,2nd, 3rd) in which the compounds 9,10 and 11 will elute from the column. Diagrams of these compounds are attached.© BrainMass Inc. brainmass.com October 25, 2018, 10:00 am ad1c9bdddf
As the solvent polarity increases, more solutes will dissolve in it. Hence, eluting power will also increase. Dichloromethane is more polar than petroleum ether and will thus ...
The order of elution is discussed for column chromatograms.
Question: Sketch the expected chromatographic elution profile (protein concentration vs elution volume) if a mixture of these proteins is applied to a weak anion exchange resin such as DEAE cellulose at a pH of 7. Label and show the salt gradient used for elution.
(A), pI 8, MW 25000
(B) , pI 10, MW 10000
(C) , pI 4 , MW 70000
(D) , pI 6, MW 5000