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    Basis for metal affinity chromatography

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    Consider immobilized metal affinity chromatography, such as a Ni2+ column.
    For this type of chromatography, describe the basis of separation of the protein components. Why do the proteins of interest bind to the resin and how are solvent conditions changed to promote elution?

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    The basis of separation for this column is the affinity certain proteins have for the metal resin. Specifically, the most common application that I know of is ...

    Solution Summary

    In this solution, we provide a short description of how metal affinity chromatography works. Information included is the basis of separation, especially in nickel columns. Also included is a description of how altering the solvent can promote the elution (dislodging) of protein from a metal affinity resin.