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Protein Purity

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How would you assess the purity of a protein?

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There are two good methods for assessing purity.

SDS-PAGE is polyacrylamide gel electrophoresis, performed in the presence of SDS (sodium dodecyl sulfate) which binds to proteins giving them a large net negative charge. Since the charges of all proteins are ...

Solution Summary

The solution to this question provides two different methods for assessing the purity of a protein. This assessment is something that would typically be asked in a 1st-year biology lab. The two solutions are methods commonly employed by scientists involved in protein purification.

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Protein Purification Questions

Hey I am did a multiweek experiment doing a protein purification of Glutamate-Oxaloacetate Transaminase (GOT). I have a few questions about my experiment.

1. How does protein purification help in the real world? What's the larger picture?

2. I preformed a GOT Assay, GDH(Glutamate Dehydrogenase) Assay, Folin-Ciocalteau Assay, passed my protein mixture through a Carboxymethylcellulose column,and SDS-Page. How do these things help me purify my target protein (GOT).

3. What is supposed to occur after each day. (Day One GOT,GDH assays, Day two Folin Assays, Day three Carboxymethylcellulose chromatography, day four SDS-page.)

4. What do I hope to learn and when is this type of experiment done?

These questions are really giving me a hard time. Please let me know if anything is unclear.

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