Purchase Solution

Protein Purity

Not what you're looking for?

Ask Custom Question

How would you assess the purity of a protein?

Purchase this Solution
Solution Summary

The solution to this question provides two different methods for assessing the purity of a protein. This assessment is something that would typically be asked in a 1st-year biology lab. The two solutions are methods commonly employed by scientists involved in protein purification.

Solution Preview

There are two good methods for assessing purity.

SDS-PAGE is polyacrylamide gel electrophoresis, performed in the presence of SDS (sodium dodecyl sulfate) which binds to proteins giving them a large net negative charge. Since the charges of all proteins are ...

Purchase this Solution
Free BrainMass Quizzes
Hemophilia: Fact or Fiction

Do you know the truth about hemophilia? Test your knowledge here.

Do You Know Your Macromolecules?

This quiz will assess your knowledge of the macromolecules that are important to living things.

Feeding Babies

Do you know the science behind feeding babies? Test your knowledge with this quiz.

The Heart

This quiz test the understanding of the heart and some of its parts. It is important to understand how the heart functions and what makes it function.

Light and Sight Vocabulary

This quiz introduces basic definitions of vocabulary related to light and how human eyes. This information is important for an understanding of sight.

View More Free Quizzes
Related BrainMass Solutions

  • Protein Purification Questions

    In addition, the SDS-PAGE will also give you idea as to the purity of your sample. In SDS-PAGE, the purity is indicated by the number of protein bands that will show in the polyacrylamide gel.

  • Reconstitution, Volume of Water, and Ovalbumin

    The purity value, however, can be important. In this case, the purity is quite high (almost 100%). Since the purity is so high, we don't need to factor in 'purity' when completing our calculation.

  • Common Biochemistry Terms

    A conjugated protein having a carbohydrate as the nonprotein component. 4. A procedure to determine the purity of a substance or the amount or activity of any particular constituent of a mixture. 5.

  • Enzymatically Recombinant cIII Deacytylases

    •This protocol yields a recombinant SIRT2 protein with high enzymatic activity and >90% purity as determined by SDS-PAGE• GST-tagged SIRT2 •SIRT2 has also been expressed using a GST fusion protein (N-terminal) using the same vector (pGEX-4T3, Amersham

  • Purity of Zn(OH)2

    101370 Purity of Zn(OH)2 The purity of a 0.287 -g samole of Zn(OH)2 is determined by titrating with a standard solution, requiring 37.8 mL.

  • Making up a standard reference solution for HPLC

    579088 Making up a standard reference solution for HPLC How can I make up 100ml of a solution containing Compound A (solid, purity of 83.7%) at 90 ug/ml and compound B (liquid, density of 1.12 and purity of 90.5%) at 30 ug/mL for HPLC reference standard

  • Hormone discovery

    Then the newly synthesized hormone can be tested for function and purity. F) The last step involves immunohistochemistry to verify the location of the hormone.

  • competitive intelligence

    The difference between the prices of Purity and ESClean is 0.13 cents. The price of Purity is twice that of ESClean.

  • Calculating percent purity from a reaction

    Assuming that the reaction goes to completion, what is he percent purity of Fe2O3 in the original sample? I balanced the equation to get Fe2O3 +3CO= 2Fe + 3CO2.

View More Related Solutions