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Protein Purity

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How would you assess the purity of a protein?

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There are two good methods for assessing purity.

SDS-PAGE is polyacrylamide gel electrophoresis, performed in the presence of SDS (sodium dodecyl sulfate) which binds to proteins giving them a large net negative charge. Since the charges of all proteins are ...

Solution Summary

The solution to this question provides two different methods for assessing the purity of a protein. This assessment is something that would typically be asked in a 1st-year biology lab. The two solutions are methods commonly employed by scientists involved in protein purification.