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Hormone discovery

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How would you experimentally verify the existence of a hormone?

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The solution gives details on experimentally determining the existence of a hormone. The solution outlines five steps and details the experiments to be done in each case to prove the existence of a hormone.

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To experimentally verify the existence of a hormone, I would do the following five steps. These steps would be performed on monkeys or willing human participants who do not mind having their organs removed, ground up and re-implanted.

A) The suspected tissue or gland of hormone origin gets ablated. This should produce deficiency symptoms in our subject.

B) The ablated tissue is ground up to produce an extract that can be injected back into the subject. The deficiency symptoms should be reversed and we should see normal function. This can pose a problem if the ...

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