Purchase Solution

Chromatography Elution

Not what you're looking for?

Ask Custom Question

Question: Sketch the expected chromatographic elution profile (protein concentration vs elution volume) if a mixture of these proteins is applied to a weak anion exchange resin such as DEAE cellulose at a pH of 7. Label and show the salt gradient used for elution.
Protein:
(A), pI 8, MW 25000
(B) , pI 10, MW 10000
(C) , pI 4 , MW 70000
(D) , pI 6, MW 5000

Purchase this Solution

Solution Summary

This solution provides a detailed explanation of the concepts which relate to this topic so that a student can really grasp the subject well. A description of how to sketch the profile is also given. This solution is a great study tool for understanding this specific subject material.

Solution Preview

Instead of sketching the graph, let's look at the concepts involved. That way, once you understand what's going on, you should be able to sketch the graphic easily yourself.

First concept:
Ion exchange resins contain charged groups. In the case of an anion exchanger such as DEAE-cellulose, the charged groups are basic. On the other hand, for a cation exchanger such as CM-cellulose, the charged groups are acidic in nature.

Additionally, cation and anion exchangers may be assessed into weak and strong exchangers depending on how well they bind the counter ions.

Second concept:
Protein samples are loaded under onto an ion exchange column at low ionic strength. This helps the proteins to bind to the column because low ionic strength promotes electrostatic interactions. Therefore, once the proteins are bound to the column, they are eluted using either a step or gradient elution of buffer with a higher ionic strength. Higher ionic strength promotes dissociation of ionic interactions and so helps facilitate the proteins coming off the ...

Purchase this Solution


Free BrainMass Quizzes
Match Elements with their Symbols

Elements are provided: choose the matching one- or two-letter symbol for each element.

General Chemistry - Classification of Matter

This test will assess your knowledge on the classification of matter which includes elements, compounds and mixtures.

Thermochemistry

The quiz helps in revising basic concepts about thermochemistry.

Organic Chemistry Naming: Alkanes

This is a quiz which is designed to assist students with learning the nomenclature used to identify organic compounds. This quiz focuses on the organic compounds called Alkanes.

Functional groups in Organic Chemistry

You will be tested on the names of functional groups in Organic Chemistry. It is very important to know the functional groups to understand Organic reactions.