Explore BrainMass
Share

Explore BrainMass

    High-performance liquid chromatography

    This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

    I recently did an experiment on high-performance liquid chromatography I am having a little trouble in the introduction. The introduction should just be a description of High-performance liquid chromatography and what I will learn from the experiment. I wrote a draft but my TA said that it could use some work. I was wondering if you could write the introduction and the what I will learn from HPLC for me so i could use as a reference to look back at. My book isn't very good. I have only opened it up to you because it seems that you have extensive experience with HPLC and you make it easy for me to understand.

    What I am looking for is
    -What is HPLC
    -What is HPLC used for
    -Advantages of HPLC
    -What I am hoping to learn from the experiment and how

    Just to clarify the experiment was the Determination of Caffeine and Sodium Benzoate in Soft Drinks by HPLC

    PS: I am just looking for about 1.5 pages not a book.

    © BrainMass Inc. brainmass.com October 10, 2019, 12:07 am ad1c9bdddf
    https://brainmass.com/chemistry/chromatography/high-performance-liquid-chromatography-279483

    Solution Preview

    Hi there,

    I've attached a good overview of HPLC. I hope this fits in with what you wanted so you can build a nice introduction to your project!

    Good luck and if you need any further help in the future with analytical, organic or inorganic chemistry, please consider requesting me.

    Introduction
    The historical development of chromatographic methodologies, especially gas chromatography, has culminated in the evolution of high-pressure liquid chromatography, HPLC. This method allows separations and measurements to made in a matter of minutes. HPLC is a popular method of analysis because it is easy to learn and use and is not limited by the volatility or stability of the sample compound. Modern HPLC has many applications including separation, identification, purification, and quantification of various compounds. HPLC is the weapon of choice for the separation of complex mixtures based on the polarity of the components.
    Underlying Principles:
    Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle. They all have a stationary phase and a mobile phase. The mobile phase flows through the stationary phase and carries the components of ...

    Solution Summary

    High performance liquid chromatography is examined.

    $2.19