Gas Chromatography Questions
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1. in gc (gas chromatography), what is the stationary phase? what is the moving phase?
2. as with the other chromatographic techniques we've studied, the polarity of the compounds affects their separation in gc. what is the other dominant factor that affects separation?
3. what would be the effect of increasing column temperature on retention times?
4. what would be the effect of increasing column temperature on the extent of separation of peaks?
5 what would be the effect of increasing carrier gas flow on retention times?
6. what would be the effect of increasing carrier gas flow on the extent of separation of peaks?
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This solution provides a detailed outline of the given pre-lab exercise questions regarding Gas chromatography.
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1. in gc(gas chromatography), what is the stationary phase? what is the moving phase?
The separation is achieved by the distribution of the mixture between two phases: one that is stationary and one that is mobile. Chromatography involves a sample or sample extract that is dissolved in a mobile phase (i.e., gas, supercritical fluid, or liquid). The mobile phase is forced through an immiscible stationary phase. The phases are such that the components of the sample/sample extract have differing solubilities and adsorption in each phase. A component that is relatively soluble in the stationary phase will spend more time traveling through it than a component that exhibits very little solubility in ...
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