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In thin layer chromatography what is the Rf's mean? How are they used? What factors affect the Rf of a compound?

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Thin layer chromatography
In thin layer chromatography what is the Rf's mean? and how are they used? and what factors affect the Rf of a compound?

Thin layer chromatography (TLC) is a technique uses to separate mixtures of substances into their components. TLC can be used to identify substances and testing the purity of compounds. (1,2) TLC have a stationary phase ( solid) and a mobile phase ( liquid). The mobile phase flows through the stationary phase and carries the components of mixture with it. (1,2) Different components travel at different rates. Silica gel is the stationary phase and the mobile phase is the liquid solvent. (1,2) The paper plate is used for the solid phse and a small drop of dye mixture is placed near the bottom of the plate. When the ...

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Thin layer chromatography (TLC) is a technique to separate mixtures of substances into their components. TLC can be used to identify substances and testing the purity of unknown compounds.

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TLC Inc Patents R&D Expenses & Amortization Expenses for 2012 and 2013

TLC, Inc. began operations in January 2012. Listed below are some transactions for 2012 and 2013.

- During 2012, $125,000 in R&D (expense) expenditures were made to develop a new product that was patented on July 1, 2012. TLC, Inc. believes the patent will provide benefits for 10 years. Legal fees incurred were $24,000 (capitalize).

- On September 1, 2012, TLC, Inc. paid MTS Co. $180,000 for its patent on a successful product. The patent has 6 remaining years in its legal life. TLC, Inc. records amortization expense of $10,000 for 2012 on the patent.

- In early January 2013, TLC, Inc. paid $20,000 in legal fees to defend the patent acquired from MTS Co. TLC's attorney was successful in the lawsuit. TLC, Inc. records amortization expense of $38,000 for 2013 on the patent and legal fees.

- During 2013, R&D (expense) expenditures of $90,000 were incurred in the development of a product. A patent was received on December 1, 2013. Legal fees paid in connection with the patent were $15,000. The economic life of the product is expected to be 5 years.

a. Prepare a partial balance sheet for TLC, Inc. as of December 31, 2013, showing the intangible assets. Provide a separate schedule for each intangible asset to support items listed on the balance sheet. TLC, Inc. amortizes its intangible assets using the straight-line method and recognizes amortization to the nearest month.

b. Prepare a schedule that calculates TLC's expenses related to the above transactions for both 2012 and 2013. Explain the calculations.

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