TLC and Azobenzene
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a) Discuss what you would observe after elution and visualization of a TLC plate having made the following mistakes in carrying out the analysis.
1. The solvent level in the developing jar was higher than the original line on which the samples were spotted.
2. Too much sample was applied to the TLC plate.
b) Over the years the two isomers of azobenzene have been designated by various terms: cis-trans, syn-anti, and E-Z. Using each set of terms, assign them to isomers of azobenzene.
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Solution Summary
The solution includes description of elution and visualization of a TLC plate, and the consequences of different types of mistakes (i.e. solvent level too high, too much sample). Isomers of azobenzene are drawn and attached as a separate document.
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a)
1). If the solvent level in the developing jar was higher than the original line on which the samples were spotted, that means that the ...
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