Why should a TLC plate be removed before the solvent before the solvent front reaches the top of the plate?
Why should the developing chamber for a TLC plate not be open to the atmosphere?
For each TLC plate in the diagrams attached, state whether the spotting method was correct or incorrect, and why?© BrainMass Inc. brainmass.com October 10, 2019, 7:46 am ad1c9bdddf
The TLC plate should be removed so the solvent does not run off the plate. If this happens, then the spots will go to the very top of the plate and other spots may also reach the top and an inaccurate analysis of the plate will ...
A few questions regarding TLC spotting are answered. This answer discusses the processes of solvents and the developing chambers for TLC plates.