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    Binding and Elution of Peroxidase

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    PI for peroxidase is 9.10

    What pH of buffers would allow for the binding and elution of the peroxidase from the resin?

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    Solution Preview

    Since you are using DEAE-sepharose, which is an anion exchanger, you need to use a buffer with a pH above the pI of ...

    Solution Summary

    The solution explains the pH of a buffer that will allow for the binding and elution of peroxidase.