Explore BrainMass
Share

Binding and Elution of Peroxidase

PI for peroxidase is 9.10

What pH of buffers would allow for the binding and elution of the peroxidase from the resin?

© BrainMass Inc. brainmass.com July 20, 2018, 4:39 pm ad1c9bdddf

Solution Preview

Since you are using DEAE-sepharose, which is an anion exchanger, you need to use a buffer with a pH above the pI of ...

Solution Summary

The solution explains the pH of a buffer that will allow for the binding and elution of peroxidase.

$2.19