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    The requirements of enzyme-linked-immunoassay.

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    With the steady developments in the field of laboratory experiments done around the world and shift from radio-immuno-assay to ELISA, present some practical implications about ELISA.

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    https://brainmass.com/biology/human-anatomy-and-physiology/the-requirements-of-enzyme-linked-immunoassay-3429

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    The conjugate used must be a highly reactive antibody coupled to an enzyme with a high turnover number.

    Alkaline phosphatase and horse-raddish-peroxidase are commonly used enzymes.

    Glyceraldehydes is a popular coupling agent but requires reactive amino groups to be a part of the enzyme as well as the ...

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