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The requirements of enzyme-linked-immunoassay.

With the steady developments in the field of laboratory experiments done around the world and shift from radio-immuno-assay to ELISA, present some practical implications about ELISA.

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The conjugate used must be a highly reactive antibody coupled to an enzyme with a high turnover number.

Alkaline phosphatase and horse-raddish-peroxidase are commonly used enzymes.

Glyceraldehydes is a popular coupling agent but requires reactive amino groups to be a part of the enzyme as well as the ...