The requirements of enzyme-linked-immunoassay.
With the steady developments in the field of laboratory experiments done around the world and shift from radio-immuno-assay to ELISA, present some practical implications about ELISA.
© BrainMass Inc. brainmass.com February 24, 2021, 2:07 pm ad1c9bdddfhttps://brainmass.com/biology/human-anatomy-and-physiology/the-requirements-of-enzyme-linked-immunoassay-3429
Solution Preview
The conjugate used must be a highly reactive antibody coupled to an enzyme with a high turnover number.
Alkaline phosphatase and horse-raddish-peroxidase are commonly used enzymes.
Glyceraldehydes is a popular coupling agent but requires reactive amino groups to be a part of the enzyme as well as the ...
$2.19