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Effect of antigen exposure on B cell response

This solution is an interpretation of an experiment that measures hapten-specific B cell response by limiting dilution assay. The explanation begins with a brief background on antigen-antibody response and the limiting dilution method to evaluate it. The effect of pre-exposure to hapten and the generation of hapten-specific B cells is detailed. The benefit of this pre-exposure on antibody response during re-exposure to hapten is the crux of the experiment in this solution. We also discuss the effects of interleukins under these experimental conditions.

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The table (which is what I assume you refer to as graph) shows the effect of memory B cell precursors. As background to explaining this assay, I will spend a few lines explaining the antibody response to the hapten TNP, for example. When the body is exposed to TNP, antigen-presenting cells (APCs) present the hapten in a recognizable form to B cells. In the presence of helper T cells, the B cells become plasma cells and secrete antibody directed against the antigen.

In the limiting dilution method, B cells are harvested from the spleens of mice and fewer and fewer B cells are cultured in the presence of the hapten to determine what minimum number (or the limiting dilution) of B cells are required to generate antibody response against the hapten. The magnitude of the response is crudely determined by measuring the number of ...

Solution Summary

This solution is an interpretation of an experiment that measures hapten-specific B cell response by limiting dilution assay. The explanation begins with a brief background on antigen-antibody response and the limiting dilution method to evaluate it. The effect of pre-exposure to hapten and the generation of hapten-specific B cells is detailed. The benefit of this pre-exposure on antibody response during re-exposue to hapten is the crux of the experiment in this solution. We also discuss the effects of interleukins under these experimental conditions.

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