Integration of the lambda phage genome into the E. Coli chromosome involves:
A Staggered cleavage of viral DNA, formation of a blunt-ended double strand break in the bacterial DNA, joining of the ends of the two DNA molecules, gap filling by DNA polymerase, and ligation of the last phosphodiester bond.
B Staggered cleavage of the viral and the bacterial DNAs, exchange of ends to generate a short heteroduplex region, and resealing.
C Double strand cleavage of the cellular DNA target site, followed by double-strand break repair, using the viral DNA as the donor.
D blunt-ended double-strand breakage in both DNAs, followed by rejoining with no heteroduplex formation.
E Double-strand cleavage of cellular DNA, annealing of a 3'-end in cellular DNA to a site in the viral DNA, replication across the viral genome, followed by another strand cleavage, and ligation of the last phosphodiester bond.
This solution answers a multiple choice question about the details of the insertion of lambda phage into the bacterial genome.
Lambda Phage and Biotechnology
Discuss lambda phage as an experimental system. How has it been used in biotechnology? Explain how it is used as a vector for the cloning of recombinant DNA. Why is it important to know about restriction enzyme sites in phage lambda DNA? How would a scientist use a restriction site map for lambda?View Full Posting Details