Drawbacks of Random Transgene Integration
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What are the benefits and drawbacks of using random transgene integration compared with homologous recombination to introduce DNA into the genome?
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Solution Summary
This solution explains what the benefits and drawbacks of using random transgene integration are when compared to the use of homologous recombination to introduce DNA into a genome.
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There are several methods for targeting the genome of an organism to either add sequence (like reporter genes or knock-ins or functional units) or remove sequence (knock-out).
Random integration of a transgene is just as it sounds, random. The DNA to be inserted can enter the genome at any point. This leads to problems of positional effect whereby the DNA ...
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