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    Drawbacks of Random Transgene Integration

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    What are the benefits and drawbacks of using random transgene integration compared with homologous recombination to introduce DNA into the genome?

    © BrainMass Inc. brainmass.com October 10, 2019, 5:07 am ad1c9bdddf
    https://brainmass.com/biology/reading-the-genome-and-gene-expression/drawbacks-random-transgene-integration-493097

    Solution Preview

    There are several methods for targeting the genome of an organism to either add sequence (like reporter genes or knock-ins or functional units) or remove sequence (knock-out).

    Random integration of a transgene is just as it sounds, random. The DNA to be inserted can enter the genome at any point. This leads to problems of positional effect whereby the DNA ...

    Solution Summary

    This solution explains what the benefits and drawbacks of using random transgene integration are when compared to the use of homologous recombination to introduce DNA into a genome.

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