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Give a brief description of each of the following:
a.Write a wild-type DNA sequence with 10 bases and under it write the sequence after a transversion and a transition has occurred. Underline and label both mutations.
b.Write a DNA sequence of 10 bases, and show how a missense mutation results in a mutant sequence. Show the sequence of the transcript and hypothetical amino acid sequence.
c.Repeat part b., but this time show the consequences of a frameshift mutation?
a.Give an example of a spontaneous mutation.
b.Describe what type of mutation results from exposure to UV light.
c.When DNA in a cell undergoes a UV radiation-induced mutation, what are the two possible options for this cell?
Describe an experiment you would do to determine if a food preservative was a mutagen.
a.In a suburb of New York, out of 135,000 people, 64 had a dominant genetic disease; 4 of these individuals had an affected parent. What is the mutation rate for this gene?
b.What does this value mean?
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Short answers with web references for given questions.
1. Give a brief description of each of the following:
a. heritable mutation
A heritable mutation is a defect which can be passed on from one generation to the next due to a defect in the DNA.
b. nutritional mutation
Nutritional mutations, also known as auxotraphic mutation, are mutations that interfere with processing of nutrients such as amino acids. For example, a mutation in the gene encoding a protein required for creating arginine in yeast is harmless if yeast are grown on media containing arginine. However, if these yeast are grown on medium lacking arginine they will die.
c. conditional mutation
Conditional mutations are mutations that are only observed under certain conditions. Temperature sensitive mutations are only observed when the temperature is increased or decreased.
2. a. Write a wild-type DNA sequence with 10 bases and under it write the sequence after a transversion and a transition has occurred. Underline and label both mutations.
DNA sequence: ATGCATGCTT
A transversion is a ...
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