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    Immunology experiment

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    An experiment that used syngeneic mice that had been immunized with ovalbumin. Experimental group: Dendritic cells were fed ovalbumin four hours prior to mixing splenic CD3+ and CD4+ mixed together (control group splenic CD3+ and CD4+ only). An hour after the cells had been mixed together they were lysed and immunoprecipitation was formed using anti-TCR Beta antibodies that had been prepared by rats. What from the following list, besides the obvious TCR beta chain, would you expect to find (possibly by IB) in the immunoprecipitate
    (Enzyme - substrate interaction is unlikely to persist long enough for them to remain in association after immunoprecipitation.)

    TCR alpha
    MHC class I alpha chain
    MHC class II beta chain
    MHC class II alpha chain
    any small GTPase

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    SOLUTION This solution is FREE courtesy of BrainMass!

    Here we are looking at the immuno-complex that assembles during antigen presentation. This is the first step in the immune reaction process and results in the identification of a foreign antigen and the initiation of the production of antibodies against it by the CD8+ and CD4+ cells. The dentritic cells are specialized antigen presenting cells and the CD4 and CD8 cells will be involved in the different MHC adhesion steps (class II and I respectively) to the antigen.

    TCR beta chain will react and bind to the following components:
    -TCR alpha
    -MHC class I alpha chain
    -MHC class II alpha chain
    -MHC class II beta chain
    -p56 lck

    The other terms listed and their activities:
    -Fyn interacts with integrins to activate Ras-signaling (enzyme)
    -SLP-76 is involved in T-cell development and activation (enzyme substrate)
    -calcineurin is a phosphatase involved in the activation of IL-2, will not bind more than transiently (enzyme)
    -small GTPase may interact with the complex but do not bind more than transiantly during GTPase activation (enzyme)
    -LAT is a transmembrane protein that operates down stream of TCR signaling (enzyme substrate)
    -IKK parallel responder to antigen presentation
    -PKC thought to link the TCR and IKK activation response to antigen presentation (enzyme)

    http://www.mcld.co.uk/hiv/?q=TCR (good diagram of the assembly of the complex)
    http://users.path.ox.ac.uk/~scobbold/tig/gloss.html (glossary of immune terms)
    http://www-immuno.path.cam.ac.uk/~immuno/part1/lec08/lec8_97.html (information on the CD8 and CD4 positive cells)
    http://en.wikipedia.org/wiki/Major_histocompatibility_complex (general overview)
    http://en.wikipedia.org/wiki/T_cell (review of T-cells)
    http://intimm.oxfordjournals.org/cgi/content/full/11/6/943 (good research article with introduction focusing on most of the listed molecules)
    http://www.bioscience.org/2004/v9/af/1390/fig7.jpg (overview picture)
    http://www.chuv.ch/ial/en/ial_t_cell_signaling_01.gif (overview pic)

    This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

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