Immunology experiment
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An experiment that used syngeneic mice that had been immunized with ovalbumin. Experimental group: Dendritic cells were fed ovalbumin four hours prior to mixing splenic CD3+ and CD4+ mixed together (control group splenic CD3+ and CD4+ only). An hour after the cells had been mixed together they were lysed and immunoprecipitation was formed using anti-TCR Beta antibodies that had been prepared by rats. What from the following list, besides the obvious TCR beta chain, would you expect to find (possibly by IB) in the immunoprecipitate
(Enzyme - substrate interaction is unlikely to persist long enough for them to remain in association after immunoprecipitation.)
TCR alpha
Lck
MHC class I alpha chain
MHC class II beta chain
MHC class II alpha chain
Fyn
SLP-76
calcineurin
CD8
CD4
CD28
any small GTPase
LAT
IKK
PKC
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Solution Summary
Several paragraphs with web references on the assembly of an immune complex upon antigen presentation. An immunology experiment detailing what types of molecules should be present is analyzed.
Solution Preview
Here we are looking at the immuno-complex that assembles during antigen presentation. This is the first step in the immune reaction process and results in the identification of a foreign antigen and the initiation of the production of antibodies against it by the CD8+ and CD4+ cells. The dentritic cells are specialized antigen presenting cells and the CD4 and CD8 cells will be involved in the different MHC adhesion steps (class II and I respectively) to the antigen.
TCR beta chain will react and ...
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