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    Table 1: Affinities of tg ARS antibodies It is possible to make transgenic (tg) mice whose B cells
    ___________________________________ express a tg heavy chain gene. An investigator recently Antibody Ka did an intriguing experiment to probe the basis for ___________________________________ humoral memory: She made single and double tg mice Ars11 10^-4 for two different heavy chains, that had been derived Ars55 10^-6 from previously characterized hybridomas. One, called ___________________________________ Ars-11, is a l o w affinity antibody for the paraazophenylarsonate (ARS) hapten when paired with endogenous kappa light chains; while the other, called Ars-55, has a high affinity for ARS (Table 1). Anti- idiotypic assays are available for each of these, allowing them to be individually tracked in various assay systems.

    Table 2: Limiting dilution analyses of The investigaor first assessed the pre-immune
    single and double tg mice frequencies of ART-reactive B cells expressing each
    ____________________________________ antibody in single and double tg mice (Table 2). She
    Knockin Mouse Primary Frequency then immunized her double tg mice with ARS10-BSA
    (per 10^5 splenic B cells) and analyzed aspects of the serum anti-ARS antibodies
    _____________________ over time (Table 3). Finally, 3 months following the
    Ars11 Ars55 primary immunization, she re-immunized the mice and
    ____________________________________ assessed their secondary responses at d5 post challenge
    Single Ars11 10 0 (Table 3).
    Single Ars55 0 10
    Double (55 puls11) 5 5

    Table 3: Characteristics of Ars-specific serum antibodies in double tg
    mice following primary and secondary immunization
    Charcteristics of Ars-specific serum antibodies
    Isotype Day post ________________________________________________
    Immunization Avg Total VH-11 VH-55
    Ka (M) Ab (mcg) (mcg) (mcg)
    0 n.d. 5 3 1
    IgM 5 10^-6 155 20 130
    21 10^-6 55 5 50
    Secondary d5 10^-6 80 5 80
    0 n.d. 3 1 1
    IgG 5 10^-4 20 10 10
    21 10^-8 200 175 10
    Secondary d5 10^-8 600 550 10

    1) What do you conclude from the data given in Table 2? Why was it important for the investigator
    to do this experiment?

    2) What do you conclude from the information about the primary response given in table 3?;

    3) What do you conclude from the information given about the memory response in Table 3?

    4) Advance a hypothesis that would explain these data and briefly outline an experiment that
    would test one aspect of your hypothesis.

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