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    Restriction sites

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    In a restriction mapping experiment using the plasmid P (ST), you cleave the circular plasmid with the restriction enzymes Xbal, Asp718, BamHI and EcoRI individually and in various combinations with each other. You obtain DNA fragments with the following sizes:

    Enzymes used size of DNA fragments (bp)

    BamHI, EcoRI, Xbal 3000, 1550
    Asp718, BamHI, Xbal 3000, 2500, 600
    Asp718, BamHI 5500, 600
    Asp718, Xbal 3600, 2500
    BamHI, Xbal 3100, 3000
    Asp718 6100
    BamHI 6100
    Xbal 6100

    Using the data above, make a map of the P (ST) plasmid indicating the distances between each restriction site for each of the four enzymes.

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    Solution Summary

    The restriction sites for mapping experiments using plasmids are determined.