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    Restriction Sites Total Length of Uncut DNA Molecules

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    You are attempting to map the restriction sites for Ecor I and BamH I on a circular DNA molecule. After conducting single digests with Ecor I and BamH I as well as a double digest, you obtain the following data:

    Ecor I BamH I Ecor I + BamH I

    fragment 1 8000bp 2500 bp 1500 bp
    fragment 2 5500 bp 2500 bp
    fragment 3 4000 bp

    What is the total length of the uncut DNA molecule?

    How many restriction sites are there for BamH I?

    Draw a diagram of the circular DNA molecule, showing the locations of the restriction sites for Ecor I and BamH I , as well as distances between them.

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    Solution Preview

    Since EcoRI gives a single fragment of 8000 bp we know that there is only 1 site for EcoRI. Therefore, the total length of the DNA molecule is 8000 bp. We can also ...

    Solution Summary

    The expert examines restriction sites for total lengths of uncut DNA molecules.