Purchase Solution

Restriction Sites Total Length of Uncut DNA Molecules

Not what you're looking for?

Ask Custom Question

You are attempting to map the restriction sites for Ecor I and BamH I on a circular DNA molecule. After conducting single digests with Ecor I and BamH I as well as a double digest, you obtain the following data:

Ecor I BamH I Ecor I + BamH I

fragment 1 8000bp 2500 bp 1500 bp
fragment 2 5500 bp 2500 bp
fragment 3 4000 bp

What is the total length of the uncut DNA molecule?

How many restriction sites are there for BamH I?

Draw a diagram of the circular DNA molecule, showing the locations of the restriction sites for Ecor I and BamH I , as well as distances between them.

Purchase this Solution
Solution Summary

The expert examines restriction sites for total lengths of uncut DNA molecules.

Solution Preview

Since EcoRI gives a single fragment of 8000 bp we know that there is only 1 site for EcoRI. Therefore, the total length of the DNA molecule is 8000 bp. We can also ...

Purchase this Solution
Free BrainMass Quizzes
Infant Development: Sleep

How much do you know about infant sleep? Test your knowledge with this quiz.

Creating a Birth Plan

Preparing for a birth and want to make sure that you're including all the right information? Use this quiz to get on the right track and check your birth plan knowledge!

Breast Milk and Breastfeeding

How much do you know about breast milk and breastfeeding? Double check your knowledge level with this quiz!

Human Anatomy- Reproductive System

Do you know your reproductive anatomy?

Pregnancy Knowledge

How much do you know about being pregnant? Test your Pregnancy IQ with this quiz!

View More Free Quizzes
Related BrainMass Solutions

  • Mechanisms of Genetic Recombination in Nature

    Furthermore, the base differences can also result in differences in length of restriction fragments when cut with an enzyme because of a gain or loss in restriction sites. These size differences are again obvious on a gel.

  • Microbiology Study Questions

    The DNA molecules of an organism in interest are isolated. The DNA molecules are then partially digested by a endonuclease restriction enzyme.

  • Restriction Enzyme Sites Frequency in DNA

    410593 Restriction Enzyme Sites Frequency in DNA Can you please explain how to do this? Thank you! How frequently in the DNA (i.e., every how many base pairs) would you find the following restriction enzyme sequences in the DNA of an organism?

  • Genetics/DNA Problems

    The best probes are those that highlight or show restriction fragments of variable length.

  • Digestion and Electrophoresis of DNA in Dogs

    137410 Digestion and Electrophoresis of DNA in Dogs The restriction enzyme Scs1 cuts at a restriction site that is found only very rarely in dog genomes. One dog, Jimmy, has two restriction sites for this restriction enzyme in his genome.

  • Using bioinformatics and DNA sequence analysis

    Their biochemical activity is the hydrolysis ("digestion") of the phosphodiester backbone at specific sites in a DNA sequence.

  • DNA Profiling using Short Tandem Repeats

    Before the discovery of STRs, Restriction fragment length polymorphism (RFLP) method was used. Using a restriction enzyme DNA collected from a source sample for eg. Blood would be digested into small pieces.

  • Human Genetics Quiz

    STSs, "sequence tagged sites" E. all of the above 16. Which of the following describes a type of polymorphism that occurs within the gene that causes Huntington's disease? A. RFLPs, "restriction fragment length polymorphisms" B.

  • To make primers and use them for site directed mutagenesis

    Complex genomes may have more spurious sites so more care is needed with, for example, mammal target DNA than bacterial target DNA. iv.

View More Related Solutions