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Restriction enzymes and modification enzyme in cloning

What is a modification enzyme / activity? Why is it important?

How are restriction enzymes used in cloning?

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Restriction enzymes usually occur in combination with one or two modification enzymes (DNA-methyltransferases) that protect the cell's own DNA from cleavage by the restriction enzyme. Modification enzymes recognize the same DNA sequence as the restriction enzyme that they accompany, but instead of cleaving the sequence, they ...

Solution Summary

Restriction enzymes usually occur in combination with one or two modification enzymes (DNA-methyltransferases) that protect the cell's own DNA from cleavage by the restriction enzyme.

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