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    Restriction enzymes and modification enzyme in cloning

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    What is a modification enzyme / activity? Why is it important?

    How are restriction enzymes used in cloning?

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    https://brainmass.com/biology/cloning/restriction-enzymes-modification-enzyme-cloning-120751

    Solution Preview

    Restriction enzymes usually occur in combination with one or two modification enzymes (DNA-methyltransferases) that protect the cell's own DNA from cleavage by the restriction enzyme. Modification enzymes recognize the same DNA sequence as the restriction enzyme that they accompany, but instead of cleaving the sequence, they ...

    Solution Summary

    Restriction enzymes usually occur in combination with one or two modification enzymes (DNA-methyltransferases) that protect the cell's own DNA from cleavage by the restriction enzyme.

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