Explore BrainMass

Explore BrainMass

    Interpreting SDS-PAGE Gels

    Not what you're looking for? Search our solutions OR ask your own Custom question.

    This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

    I have analysed some fish proteins using SDS-PAGE. My results varied but some showed a similar banding pattern between samples. My question is what does two similar banding patterns suggest?

    © BrainMass Inc. brainmass.com March 4, 2021, 8:17 pm ad1c9bdddf

    Solution Preview


    I am going to assume that your were analyzing fish proteins from different fish.

    Background about SDS-PAGE

    Polyacrylamide gel electrophoresis (PAGE) is a simple technique for separating bio-macromolecules such as proteins and DNA on the basis of size (ie molecular weight). In your experiment, higher molecular weight proteins will move slower through the gel matrix and be found near the top of th gel while lower molecular weight proteins will migrate faster and be found closer to the bottom of the gel. The SDS component (sodium dodecylsulphate) will ensure ...

    Solution Summary

    When doing a comparative analysis of proteins from similar organisms, what do similar bands on an SDS-PAGE gel indicate?