Explore BrainMass

Explore BrainMass

    five step protocol

    Not what you're looking for? Search our solutions OR ask your own Custom question.

    This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

    Using a five-step protocol, you have purified an enzyme to the point where the specific activity remains constant when you perform any additional purification steps. When you run the purified enzyme on a gel filtration chromatography column, you find it has a molecular mass of 50 kD. However, when you run the purified enzyme on a SDS-PAGE gel, you obtain two different sized peptides one 30 kD and the other 20 kD. What could be the possible explanations for this result? (KAS)

    © BrainMass Inc. brainmass.com November 30, 2021, 5:14 am ad1c9bdddf

    Solution Summary

    This solution assists in interpreting the results of an enzyme purification.