Explore BrainMass
Share

five step protocol

This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

Using a five-step protocol, you have purified an enzyme to the point where the specific activity remains constant when you perform any additional purification steps. When you run the purified enzyme on a gel filtration chromatography column, you find it has a molecular mass of 50 kD. However, when you run the purified enzyme on a SDS-PAGE gel, you obtain two different sized peptides one 30 kD and the other 20 kD. What could be the possible explanations for this result? (KAS)

© BrainMass Inc. brainmass.com March 21, 2019, 10:57 pm ad1c9bdddf
https://brainmass.com/biology/research-methods-and-experimental-design/protocol-enzyme-purification-438576

Solution Summary

This solution assists in interpreting the results of an enzyme purification.

$2.19