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    size exclusion chromatography and SDS-PAGE

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    Describe whether, and how, size exclusion chromatography and SDS-PAGE can be used to determine the molecular weight and oligomeric status of a protein. Indicate whether it is necessary to compare the protein's mobility relative to standards of known molecular weight.

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    In size-exclusion chromatography, molecules are separated according their physical size, not by molecular weight. however; the molecular weight can be calculated by relating the hydrodynamic volume (space taken up by the molecule in solution) to the hydrodynamic volume of polystyrene, which must be used as a standard with this technique. As the relationship between ...

    Solution Summary

    This solution describes how size exclusion chromatography and SDS-PAGE can be used to determine the molecular weight and oligomeric status of a protein.

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