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    Gel-Filtration / Size Exclusion Chromatography

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    Why do smaller molecules elute after large molecules when a mixture of proteins is passed through a size-exclusion (gel filtration) column?

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    https://brainmass.com/chemistry/chromatography/gel-filtration-size-exclusion-chromatography-325221

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    Why do smaller molecules elute after large molecules when a mixture of proteins is passed through a size-exclusion (gel filtration) column?

    In gel-filtration chromatography (also termed Size Exclusion Chromatography) a mixture of different sized molecules (for example: proteins) are applied to the top of a column. The column itself consists of millions and millions of small, porous ...

    Solution Summary

    Gel-filtration and size exclusion chromatography is examined. A full solution is provided.

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