Explore BrainMass

Preparing solutions for biochemical experiments

This content was STOLEN from BrainMass.com - View the original, and get the already-completed solution here!

In preparation for a protein purification experiment, you have been asked to prepare 100 mL of an aqueous solution of Tween20. The solution should have a final concentration of 4.25% (v/v) Tween 20. There is a bottle of Tween 20 (100% v/v) on the lab shelf. Describe the method that you will use to prepare the solution.

© BrainMass Inc. brainmass.com October 25, 2018, 8:59 am ad1c9bdddf

Solution Preview

To answer this problem, you need to understand the concept of expressing concentration as a percentage. In this case, the desired concentration is expressed as "% (v/v)", where the "v/v" means "volume of solute / total volume".

The official formula for calculating the volume percent is :
Volume percent = (volume of solute) / (volume of solution) ) x 100%

What is a solute? A solute is the "substance" that makes up your solution. The solute is dissolved in or mixed into a solvent to create a solution. In this case, the "solute" is a detergent called Tween 20, and the solvent is water.

Watchpoint: A COMMON MISTAKE made by ...

Solution Summary

The solutions applies volume percent and solution chemistry to calculate the volume of solute needed to prepare a laboratory solution

See Also This Related BrainMass Solution

Microbiology Questions

- You have a rabbit with an unknown illness. Apply Koch's postulates to prove that a specific bacterial species is responsible for the rabbit's illness. Identify all four postulates in your answer and describe how you would set up experiments to specifically address each postulate.

- List and describe 2 distinguishing features of prokaryotes and eukaryotes (ie: not in common) and 2 features that prokaryotes and eukaryotes have in common.

- Briefly describe the debate between the theory of spontaneous generation and the theory of biogenesis. Explain how Louis Pasteur's experiment with the S-neck flasks in the 1800s resolved this debate. Include a detailed description of Pasteur's experiment and an analysis of how it successfully proved one theory over the other.

- Explain the code of DNA and how the code gets translated into an amino acid sequence. Be sure to explain each step fully.

View Full Posting Details