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    Understanding Recombination in Bacteria.

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    A transformation experiment is performed with a donor strain that is resistant to four drugs: A, B, C, D. The recipient is sensitive to all four drugs. The treated recipient-cell population is divided up and plated on media containing various combinations of the drugs. The results are shown in the table below.

    Drug # colonies
    NONE 10000
    A 1156
    B 1148
    C 1161
    D 1139
    AB 46
    AC 640
    AD 942
    BC 51
    BD 49
    CD 786
    ABC 30
    ABD 42
    ACD 630
    BCD 36
    ABCD 30

    (a) One of the genes obviously is quite distant from the other three, which appear to be tightly (closely) linked. Which is the distant gene?

    (b) What is the probably order of the three tightly linked genes?

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    Solution Summary

    Recombination in bacteria is examined. The various combination of the drugs are determined.