Six mutants in the rII region of phage T4 were independently isolated. Recombination to produce wild-type progeny occurred between all of these mutants. Pairwise multiple infections of E.coli strain K12 were made with the results shown below. (The number of phage particles used in each multiple infection was approximately the same, and was constant among experiments).
(a) Fill in a complementation table. Use "+" for complementation and "0" for no complementation.
(b) How many genes are affected among all these mutant strains?
(c) Which mutants have mutations in the same gene(s)?
View attachment below for full solution.© BrainMass Inc. brainmass.com October 24, 2018, 5:17 pm ad1c9bdddf
In the simplest terms, phages infect bacteria. These infections are called plaques on a plate of growing bacteria (E. coli K12 in this case). While the number of plaques varies, we can say here than any non-zero value represents recombination, since they're all in the same sort of range. If, however there was a plate of E. coli that only had a few plaques, we might suspect that there had ...
Six mutation regions of phages are determined. A pairwise multiple infections of E. coli strains are examined.
Prokaryotic Genetic Recombination and Types of Operons
Discuss the two different types of operons found in bacterial genomes (inducible operons and repressible operons) and describe how they work.
Then, describe the following three different forms of prokaryotic genetic recombination: conjugation, transformation, and transduction.
Hypothesize how recombination might adversely affect the functioning of an operon such as the lac operon or the trp operon. What might be the metabolic implications for an E. coli cell that experiences a disruption of one of these operons?View Full Posting Details