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Isolating DNA

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5. DNA is precipitated out of a water solution by adding an alcohol. Why do think DNA is less soluble in alcohol than in water?

6. Draw the structures of the 4 nucleotide bases found in DNA. What features do they have in common? How are they different?

7. Hydrogen bonds are responsible for holding the two DNA strands together. Show by drawing which bases always pair up in a DNA strand.

8. Suggest why DNA molecules that are rich in cytosine and guanine decompose at higher temperatures than DNA rich in adenine and thymine.

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Questions regarding DNA and nucleotide bases are provided. The expert determines why you think DNA is less soluble in alcohol than in water.

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Isolating DNA
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5. DNA is precipitated out of a water solution by adding an alcohol. Why do think DNA is less soluble in alcohol than in water?

Answer - In water DNA produces non-covalent bonds with the highly polar water molecules. Individually these bonds are weak but there are so many of them and the DNA chain is so long, it 'glues' the water ...

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