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    Western, Southern, and Northern Blotting Procedures

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    What is the difference between a Southern blot, a Northern blot, and a Western blot?
    Include a basic overview of the procedures.

    © BrainMass Inc. brainmass.com May 24, 2023, 12:51 pm ad1c9bdddf

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    Southern blotting was the first type of blot developed, and it was named after its inventor, E.M. Southern.

    Southern blotting involves DNA.

    Northern blotting involves RNA.

    Western blotting involves protein.

    All three techniques involve isolating the macromolecules of interest (RNA, DNA or protein) away from the rest of the cellular constituents. Genomic (and mitochondrial) DNA must be cut into smaller pieces ...