Explore BrainMass

Western, Southern, and Northern Blotting Procedures

This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

What is the difference between a Southern blot, a Northern blot, and a Western blot?
Include a basic overview of the procedures.

© BrainMass Inc. brainmass.com March 21, 2019, 10:02 am ad1c9bdddf

Solution Preview

Southern blotting was the first type of blot developed, and it was named after its inventor, E.M. Southern.

Southern blotting involves DNA.

Northern blotting involves RNA.

Western blotting involves protein.

All three techniques involve isolating the macromolecules of interest (RNA, DNA or protein) away from the rest of the cellular constituents. Genomic (and mitochondrial) DNA must be cut into smaller pieces ...