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    Restriction Enzyme DNA

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    The restriction enzyme ECOR I cuts DNA leaving 5' overhangs, Pst I leaves 3' overhangs after cutting DNA and SMA I leaves blunt ends on the fragments it produces.Indicate wheter DNA ligase can join the following pieces of DNA.
    a) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was also cut with Ecor I .

    b) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was cut with Pst I.

    c) a fragment of human DNA that was generated by cutting with Sma I and a plasmid DNA that was also cut with Sma I.

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    https://brainmass.com/biology/dna-chromosomes-and-genomes/restriction-enzyme-dna-73969

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    The restriction enzyme ECOR I cuts DNA leaving 5' overhangs, Pst I leaves 3' overhangs after cutting DNA and SMA I leaves blunt ends on the fragments it produces. Indicate wheter DNA ligase can join the following pieces of DNA.

    a) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was also cut with Ecor I .

    b) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was cut with Pst I.

    c) a fragment of human DNA that was generated by cutting with Sma I and a plasmid DNA that was also cut with Sma I.

    Solution:

    Restriction enzyme ECOR I cuts DNA, leaving 5' overhangs:

    Recognition sequence is

    5′---GAATTC

    3′---CTTAAG

    Note: Arrow marks indicate that the cut site (Target nucleotide sequence) for enzyme.

    Restricted fragment will be.....

    5′---G AATTC ---3′

    3′---CTTAA G ...

    Solution Summary

    The fragments of human DNA that was generated by cutting with Ecor I and a plasmic DNA that was cut with Pst I is examined. DNA ligase for pieces of DNA are analyzed.

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