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Protein analysis by polyacrylamide gel electrophoresis

I have used a polyacrylamide gel in a protein analysis of fish samples. My question is why is this used instead of an agarose gel. and what is the purpose of the two reagents ammonium persulphate and TEMED when used to make polyacrylamid gels? Hope you can help.

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Agarose is a polysaccharide extracted from seaweed. It is typically used at concentrations of 0.5 to 2%. The higher the agarose concentration the "stiffer" the gel. Agarose gels have a large range of separation, but relatively low resolving power. It is commonly used during electrophoresis to separate large size molecule like DNA. By varying the concentration of ...

Solution Summary

Polyacrylamide is a cross-linked polymer of acrylamide. The length of the polymer chains is dictated by the concentration of acrylamide used, which is typically between 3.5 and 20%. Polyacrylamide gels have a rather small range of separation, but very high resolving power.

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