Explore BrainMass

Carbon Tracking in TCA Cycle

This content was STOLEN from BrainMass.com - View the original, and get the already-completed solution here!

C14-Glucose labeled at carbon 5 enters glycolysis, is made into pyruvate, then acetyl CoA, and enters the TCA cycle. How much of the C14 will be released as CO2 during the second turn of the TCA cycle?

© BrainMass Inc. brainmass.com October 24, 2018, 10:15 pm ad1c9bdddf

Solution Preview

Get out your textbook and look at the structures as I walk you through the process of coming up with the solution.

What happens to the [14C]-Glucose labeled at carbon 5 as it goes through glycolysis?

The important point is that when the six carbon fructose-1,6-bisphosphate splits into DHAP and G3P, and that when DHAP converts into another G3P, that C5 (and C2 for that matter) both end up being the middle carbon in G3P. You can go through the individual steps on your own to confirm this, but it can be easy to remember:

C6 of glucose gets phosphorylated, right?
When it becomes fructose, the same C6 is phosphorylated.
Then, fructose gets phosphorylated on C1 too. Therefore, both ends are phosphorylated, C1 and C6.
When you get to the two G3P molecules, just remember that the carbons with the phosphates on them are the same carbons that ...

See Also This Related BrainMass Solution

Definition of Life from a Biological Perspective

Find any media piece (article, video, presentation, song, or other) related to scientific method and describe how it helped to better understand how the scientific method is used to create hypotheses and experiments.

Find a media piece that recognizes the fundamental concepts of chemistry in biology and describe how it helps to better understand how fundamental concepts of chemistry affect biology.

Find a media piece that describes the energy metabolism and how it helps to better understand the energy metabolism of cells.

Find any media piece that compares structures and functions of different cell types and describe how it helped better compare structures and functions of different cell types.

What is the meaning of life after finding this information.

View Full Posting Details