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Comparing Rates of CO2 Generation

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The relative metabolic activities in an organism of glycolysis + the citric acid cycle vs. the pentose phosphate pathway + gluconeogenesis can be measured by comparing rates of 13CO2 generation upon administration of glucose labeled with 13C at C1 with that of glucose labeled at C6. Explain why this is possible.

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Solution Summary

This in-depth solution explores why relative metabolic activities of an organism can be measured by comparing rates of 13CO2 generation. Each pathway is explained in detail and the number of 13CO2 is counted.

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USING 13C TO MEASURE THE RELATIVE ACTIVITIES OF GLYCOLYSIS/TCA VS. PENTOSE PHOSPHATE/GLUCONEOGENESIS

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The relative metabolic activities in an organism of glycolysis + the citric acid cycle vs. the pentose phosphate pathway + gluconeogenesis can be measured by comparing rates of 13CO2 generation upon administration of glucose labeled with 13C at C1 with that of glucose labeled at C6. Explain why this is possible.

Response:

In order to figure this out, we must look at the reactions in each of the two sets of pathways that release the CO2. Let's start with the ...

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