What is the evidence that fructose bisphosphate aldolase is a Class I Aldolase?
Response: Fructose bisphosphate aldolase (FBPA) belongs to the Class I Aldolase family because it catalyzes the cleavage of fructose 1,6-bisphosphate (FBP) through an intermediate Schiff base between the conserved and invariant active site Lysine (K) and the C2 carbonyl group on FBP. Once the Schiff base is formed, a glutamate residue acts as a proton donor to cleave the C3-C4 bond forming the two products, dihydroxyacetone phosphate (DHAP) and glyceraldehyde C-C bond cleavage. If you want, you can go to http://www.chem.uwec.edu/Webpapers2005/lawrenmj/pages/page_1.html to see a short summary of the basic mechanism of this enzyme.
If you go to ...
This solution describes fructose bisphosphate aldolase.