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The Enzymes of Glycolysis

What are the various strategies used to regulate glycolysis (do not include glycogen phosphorylase in this question), but include how various inhibitors and activators function, how a highly endergonic reaction is facilitated, and what the roles of isozymes are.

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Glycolysis is a tightly regulated event in the cell. Glucose flux is regulated to achieve constant ATP levels by regulation of three glycolytic enzymes. This regulation is allosteric in that the concentration of key metabolites that reflect the cellular balance between ATP production and consumption is in consideration.

Coordinated Regulation of Glycolysis and Gluconeogenesis:

Three glycolytic enzymes are subject to allosteric regulation [actually 4 if you include glycogen phosphorylase]: hexokinase IV, phosphofructokinase-1(PFK-1), and pyruvate kinase.

Hexokinase IV (glucokinase) is sequestered in the nucleus of the hepatocyte, but is released when the cytosolic glucose concentration rises. Hexokinase is an isoenzyme.

PFK-1 is allosterically ...

Solution Summary

The solution provides a detailed explanation of the strategies for regulating glycolysis. Included are explanations of inhibitors, activators, and isozymes. Detailed diagrams are also provided as attachments.