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Concentrations and Dilutions

There is 100 mg of protein dissolved in 100 mL water. Then 10 microliters of that solution is dissolved in 990 microliters of water for total volume of 1.0 mL. To calculate the concentration of protein is it 10/990 or 10/1000? And does the original concentration of solution even matter when calculating the solution concentration?

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You will have to use the 10/1000 ratio for your calculation. The equation you want ...