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Determination of the Titer of B-D-Galactosidase Using the ELISA

The purpose of the experiment that took place was to determine the titer of B-D-galactosidase using the enzyme-linked immunosorbent assay. given the results of the experiment, how do you determine the titer, and what do these results mean? The raw data (results obtained from the plate reader) are attached in the excel file. I found the of the inverse of each antibody and graphed it, but I am unsure what to do after that, and I dont even know if I'm on the right track. A classmate told me that my results were inconclusive because my points are inconsistent. If so, why could this have happened?


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The purpose of determining the titer is to determine what the minimum value of OD that should be used in the ELISA experiments. Normally the titer is determined by determining the mean of the negative controls plus 2 or 3 standard deviations above the mean. The purpose of graphing the inverse of each antibody to the OD is determine which antibody concentration is above the value that you determined above. For example, if 0.4 is equal to the mean of the negative control plus 2 or 3 standard deviations, then you use the antibody concentration that has a OD value above 0.4. This allows you to trust the signal that you receive is really coming from your antibody interacting with antigen and not ...

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The expert provides a determination of the Titer of B-D-Galactosidase using the ELISA.