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How to create serial dilutions from a stock solution.

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2,3,7,8-Tetrachloro-dibenzo-p-dioxin (TCDD) is pre-made at 32pg/ul = 100nM. The total volume is 200 uL. I need to prepare a working solution of this compound at 30nM per 1mL, then 3nM per 1mL, and finally at 0.3nM per 1mL.

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Solution Preview

Hi there,

Given that you would like to make as much of the 30 nM solution as possible with the 100 nM you are given, please follow the following calculations.

The dilution factor will be 1:3.33

Since you want to use all of the 200 ul of the 100 nM solution, ...

Solution Summary

This solution will describe how to create serial dilutions of a stock solution to create final working concentrations.

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