Explore BrainMass

Explore BrainMass

    How to create serial dilutions from a stock solution.

    Not what you're looking for? Search our solutions OR ask your own Custom question.

    This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

    2,3,7,8-Tetrachloro-dibenzo-p-dioxin (TCDD) is pre-made at 32pg/ul = 100nM. The total volume is 200 uL. I need to prepare a working solution of this compound at 30nM per 1mL, then 3nM per 1mL, and finally at 0.3nM per 1mL.

    © BrainMass Inc. brainmass.com March 6, 2023, 2:45 pm ad1c9bdddf

    Solution Preview

    Hi there,

    Given that you would like to make as much of the 30 nM solution as possible with the 100 nM you are given, please follow the following calculations.

    The dilution factor will be 1:3.33

    Since you want to use all of the 200 ul of the 100 nM solution, ...

    Solution Summary

    This solution will describe how to create serial dilutions of a stock solution to create final working concentrations.