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    Protein assay/ protein concentration

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    Given data from a protein assay with Coomassie blue dye solution taken and graphed at ABS @ 595 nm with BSA concentrations (ug/ml); 1000, 500, 250, 125, 62.5, 31.5....etc what might explain why the ABS vs BSA concentration data is linear at the lower BSA concentration and non-linear at higher [BSA]?

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    The most likely reason for the non-linearity of the standard curve is the fact that the assay is based on the binding of the dye reagent to the protein (BSA). This binding ...

    Solution Summary

    The expert examines protein assays and protein concentration.