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Reading Scientific Research Papers

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I am reading up on a research paper. Can someone explain how using a luciferase reporter gene by being positioned next to the PPRE-containing fatty acyl CoA oxidase gene promoter? Was used to assess the effect of X protein since it was not in the knockout mice? I would appreciate it if it is short and simple so I can understand it.

The paragraph of interest is;

The authors then examined how X ( a co-activator for gene transcription) interacts with other proteins that are known to be regulated by a transcription factor called PPARG. PPAR proteins are nuclear receptors that act as transcription factors that exert their transcriptional activation effects by binding to target sequences or response elements called PPRE's.To assess the effect of of protein X, a luciferase reporter gene was positioned next to the PPRE-containing fatty acyl CoAoxidase gene promoter
The cells used are fibroblast cells from knock out mice. so have no protein X

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Solution Preview

Thanks for letting me work on your post. Here is my explanation:

The idea here is the luciferase reporter gene is under the regulation of PPRE-containing fatty acyl ...

Solution Summary

The expert examines reading scientific research papers. Fatty acyl CoA oxidase gene promoters are examined.

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2). Briefly how does research articles differs from other types of informative articles, such as magazine features or research papers, which typically address the topic in a general scope as a means of introduction? Provide an example.

3). As with any type of targeted writing, there is a protocol to follow when writing a research article in terms of layout. What keywords or indicators in the title to help one to identify this type of article (Research Article)? Other words what should the title provide to help one to identify the type of scholarly article that they have selected? Provide an example.

4).What keywords or indicators in the abstract portion of the research article to one identify this type of article (Research Article)? Other words what should be in the abstract portion of a research article to help one identify the type of scholarly article they have selected? Provide an example.

5).Provide a general overview of a Review Article. Summarize the main points based on this type of scholarly article (e.g., for a research article, summarize research questions, population, findings, limitations, and recommendations for future study).

Minimum of 350 words and least two scholarly references preferably one from the American Psychological Association manual to answer this posted question. Limit the use of direct quotes, and do your best to critically evaluate and synthesize the solution that you are presenting to this posted question in your own words wherever possible. Make sure to include in-text citations and a reference list as appropriate. Use proper APA formatting; including in-text citations is necessary.

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