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    To calculate protein concentration with given absorbance

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    Question1.
    The data below was obtained in an experiment to determine the protein concentration of two unknown solutions. Analyse the data, plot a suitable graph and determine the protein concentrations of the unknowns

    Protein standard (mg cm-3) Absorbance at 540nm
    0 0.00
    1 0.122
    2 0.243
    3 0.365
    4 0.468
    5 0.588

    Unknowns Absorbance in triplicate
    1 0310, 0.318, 0.322
    2 0.444, 0.423, 0.443

    Question 2.
    State two things which may result in error in this type of experiment?

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    https://brainmass.com/biology/research-methods-and-experimental-design/to-calculate-protein-concentration-with-given-absorbance-88432

    Solution Preview

    Please see the attached file.

    Equation for the value given is : y = 0.122x

    Mean of the given absorbance : (.31 + .318 + .322)/3 = 0.316

    Protein content for this absorbance ...

    Solution Summary

    Protein concentration in the given solution can be known through photospectrometric method. A direct correlation exists between protein concentration and its absorbance.

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