The data below was obtained in an experiment to determine the protein concentration of two unknown solutions. Analyse the data, plot a suitable graph and determine the protein concentrations of the unknowns
Protein standard (mg cm-3) Absorbance at 540nm
Unknowns Absorbance in triplicate
1 0310, 0.318, 0.322
2 0.444, 0.423, 0.443
State two things which may result in error in this type of experiment?
Please see the attached file.
Equation for the value given is : y = 0.122x
Mean of the given absorbance : (.31 + .318 + .322)/3 = 0.316
Protein content for this absorbance ...
Protein concentration in the given solution can be known through photospectrometric method. A direct correlation exists between protein concentration and its absorbance.
Using Beer's Law to Calculate Glucose Concentrations
I have a series of protein free patient blood samples at time; 0, 30, 60, 90, 120, 180 mins. I also have a standard sample. I have measured the absorbance of these samples; 0.655, 0.717, 0.689, 0.430, 0.852, 0.477 respectively. Standard sample 0.760 The glucose concentration in the standard test tube is 0.278 mmole/L. Using Beers Law, how do i calculate the glucose concentrations each of the timed samples?View Full Posting Details