Explore BrainMass

To calculate protein concentration with given absorbance

This content was STOLEN from BrainMass.com - View the original, and get the already-completed solution here!

The data below was obtained in an experiment to determine the protein concentration of two unknown solutions. Analyse the data, plot a suitable graph and determine the protein concentrations of the unknowns

Protein standard (mg cm-3) Absorbance at 540nm
0 0.00
1 0.122
2 0.243
3 0.365
4 0.468
5 0.588

Unknowns Absorbance in triplicate
1 0310, 0.318, 0.322
2 0.444, 0.423, 0.443

Question 2.
State two things which may result in error in this type of experiment?

© BrainMass Inc. brainmass.com October 24, 2018, 8:07 pm ad1c9bdddf

Solution Preview

Please see the attached file.

Equation for the value given is : y = 0.122x

Mean of the given absorbance : (.31 + .318 + .322)/3 = 0.316

Protein content for this absorbance ...

Solution Summary

Protein concentration in the given solution can be known through photospectrometric method. A direct correlation exists between protein concentration and its absorbance.

See Also This Related BrainMass Solution

Using Beer's Law to Calculate Glucose Concentrations

I have a series of protein free patient blood samples at time; 0, 30, 60, 90, 120, 180 mins. I also have a standard sample. I have measured the absorbance of these samples; 0.655, 0.717, 0.689, 0.430, 0.852, 0.477 respectively. Standard sample 0.760 The glucose concentration in the standard test tube is 0.278 mmole/L. Using Beers Law, how do i calculate the glucose concentrations each of the timed samples?

View Full Posting Details