To calculate protein concentration with given absorbance
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Question1.
The data below was obtained in an experiment to determine the protein concentration of two unknown solutions. Analyse the data, plot a suitable graph and determine the protein concentrations of the unknowns
Protein standard (mg cm-3) Absorbance at 540nm
0 0.00
1 0.122
2 0.243
3 0.365
4 0.468
5 0.588
Unknowns Absorbance in triplicate
1 0310, 0.318, 0.322
2 0.444, 0.423, 0.443
Question 2.
State two things which may result in error in this type of experiment?
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Solution Summary
Protein concentration in the given solution can be known through photospectrometric method. A direct correlation exists between protein concentration and its absorbance.
Solution Preview
Please see the attached file.
Equation for the value given is : y = 0.122x
Mean of the given absorbance : (.31 + .318 + .322)/3 = 0.316
Protein content for this absorbance ...
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