Share
Explore BrainMass

To calculate protein concentration with given absorbance

Question1.
The data below was obtained in an experiment to determine the protein concentration of two unknown solutions. Analyse the data, plot a suitable graph and determine the protein concentrations of the unknowns

Protein standard (mg cm-3) Absorbance at 540nm
0 0.00
1 0.122
2 0.243
3 0.365
4 0.468
5 0.588

Unknowns Absorbance in triplicate
1 0310, 0.318, 0.322
2 0.444, 0.423, 0.443

Question 2.
State two things which may result in error in this type of experiment?

Solution Preview

Please see the attached file.

Equation for the value given is : y = 0.122x

Mean of the given absorbance : (.31 + .318 + .322)/3 = 0.316

Protein content for this absorbance ...

Solution Summary

Protein concentration in the given solution can be known through photospectrometric method. A direct correlation exists between protein concentration and its absorbance.

$2.19