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DNA cloning

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1- The genes for both the alpha-and beta-globin chains of hemoglobin contain introns (i.e., they are split genes). How would this fact affect your plans if you wanted to introduce the gene for alpha-globin into a bacterial plasmid and have the bacteria produce alpha-globin?

2- Bacteria and yeast are known not to have prions. What does this fact have to do with the popularity of expressing mammalian proteins using bacterial vectors?

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Short answers to two DNA cloning questions are provided in the solution.

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1. Bacteria are unable to process introns therefore in order to express alpha-globin in a bacterial cell the introns must be removed ...

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