When quantifying the activity of an enzyme, does it matter wheter you measure the appearence of a product or the dissapearence of a reactant?© BrainMass Inc. brainmass.com September 24, 2018, 9:45 pm ad1c9bdddf - https://brainmass.com/biology/enzyme-catalysts-and-reactions/quantifying-the-activity-of-an-enzyme-36399
Question: When quantifying the activity of an enzyme, does it matter whether you measure the appearance of a product or the disappearance of a reactant?
Response: No, it shouldn't make a bit of difference. A biochemist will need to find some way of measuring either (a) the appearance of product or (b) disappearance of the reactant. Since the two are directly correlating and one is completely dependent on the other, measuring ...