When quantifying the activity of an enzyme, does it matter wheter you measure the appearence of a product or the dissapearence of a reactant?© BrainMass Inc. brainmass.com October 24, 2018, 6:16 pm ad1c9bdddf
Question: When quantifying the activity of an enzyme, does it matter whether you measure the appearance of a product or the disappearance of a reactant?
Response: No, it shouldn't make a bit of difference. A biochemist will need to find some way of measuring either (a) the appearance of product or (b) disappearance of the reactant. Since the two are directly correlating and one is completely dependent on the other, measuring ...
Protein Purification Questions
Hey I am did a multiweek experiment doing a protein purification of Glutamate-Oxaloacetate Transaminase (GOT). I have a few questions about my experiment.
1. How does protein purification help in the real world? What's the larger picture?
2. I preformed a GOT Assay, GDH(Glutamate Dehydrogenase) Assay, Folin-Ciocalteau Assay, passed my protein mixture through a Carboxymethylcellulose column,and SDS-Page. How do these things help me purify my target protein (GOT).
3. What is supposed to occur after each day. (Day One GOT,GDH assays, Day two Folin Assays, Day three Carboxymethylcellulose chromatography, day four SDS-page.)
4. What do I hope to learn and when is this type of experiment done?
These questions are really giving me a hard time. Please let me know if anything is unclear.View Full Posting Details