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enzymatic activity

Design and carry out an experiment in which you will test the effects of acidic fluid on enzymatic activity.

1. What question are you asking? Clearly state your hypothesis. Describe your IV and DV.
2. Design your experiment. Provide a detailed account of the materials and methods used to conduct the experiment. Also include the methods for data collection and analysis.
3. Conduct the experiment and record your results. Make a data table that summarizes your findings. You could include what you observed - "which samples showed bubbling?
4. Use your knowledge about enzymes to interpret your results. It may be necessary for you to refer to your textbook. What effect does the acid have on the enzyme?
5. Was your hypothesis supported? What is your conclusion?

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INTRODUCTION:

Enzyme are protein that speed up a reaction. (1) They are sometimes referred to as catalyst. An enzyme (catalyst) speeds up a reaction by lowering the activation energy of a chemical reaction. (1) An enzyme works by making the chemical reaction work faster. A chemical reaction must involve the breaking of a bond in the reactant and the forming of new bond in the product. The breaking and forming of bonds requires energy. (1) The reactant molecule must absorb sufficient energy for their bond to break. The initial requirement of energy for breaking the bond of the reactant in a chemical reaction is know as the "activation energy, -"G. (1) In the reaction A + B must absorb sufficient energy to overcome the activation energy, which is like a hill so that the product C +D is reached. The activation energy is like a hill that must be A +B, the reactant must overcome to reached C +D, the product. Then, there are enzymes that come into play. They act to lower the activation energy or make the hill lower so that the reactant (A +B ) can proceed to C +D.

Lets look closely at the enzyme catecholase. Catecholase is an enzyme in fruit and vegetables that reacts to the substrate (reactant) catechol and oxygen. (2, 3) In the presence of oxygen, the substrate catechol is oxidized to the molecule Benzoquinone. The Benzoquinone molecule form "red and brown melanoid pigments that causes the darkening in bruished fruit" (2,4)

pH is a measure of hydrogen ion concentration. It is one of the most important factor in the determining the activity of the ...

Solution Summary

Enzyme are protein that speed up a reaction. (1) They are sometimes referred to as catalyst. An enzyme (catalyst) speeds up a reaction by lowering the activation energy of a chemical reaction. (1) An enzyme works by making the chemical reaction work faster. A chemical reaction must involve the breaking of a bond in the reactant and the forming of new bond in the product. The breaking and forming of bonds requires energy. (1) The reactant molecule must absorb sufficient energy for their bond to break. The initial requirement of energy for breaking the bond of the reactant in a chemical reaction is know as the "activation energy, -"G. (1) In the reaction A + B must absorb sufficient energy to overcome the activation energy, which is like a hill so that the product C +D is reached. The activation energy is like a hill that must be A +B, the reactant must overcome to reached C +D, the product. Then, there are enzymes that come into play. They act to lower the activation energy or make the hill lower so that the reactant (A +B ) can proceed to C +D.

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