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Maximal Enzyme Velocity and Turnover Number

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The enzyme carbonic anhydrase is found in erythrocytes, and has a molecular mass of approximately 30000 Da. The hydration reaction catalyzed by this enzyme is reversible, and plays a role in the transport of CO2 from the tissues to the lungs.

H2O + CO2 <-> H2CO3

If 20 micrograms of pure enzyme catalyzes the hydration of 0.50 g of Co2 in 1 minute, at 37 degrees Celsius, under optimal conditions, what is the turnover number (kcat) for carbonic anhydrase?

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Solution Summary

This problem and solution provide a step by step example of an enzyme problem of the type that often appears on undergraduate examinations and laboratory reports. The solution includes a step by step explanation of how to approach this type of problem, as well as the complete answer to the question

Solution Preview

The definition of turnover number (or kcat) is shown below:

kcat = Vmax/([E]total)

In part one of the ...

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