Real Time Polymerase Chain Reaction

Related BrainMass Solutions

• Polymerase Chain Reaction - PCR

  45867 Polymerase Chain Reaction - PCR I am legally blind and therefore, I cannot see the board in the classroom, and I also cannot read regular print because my vision is too low.

• Amplify a Stretch of DNA

  The enzyme typically used for PCR is a thermostable DNA polymerase such as Taq polymerase. The solution assists with amplifying a stretch of DNA with the given sequence using the polymerase chain reaction.

• DNA-Sequencing

  For DNA sequencing using the chain-termination method (also termed Sanger or dideoxy method), four reactions are performed, each using theDNA to be analyzed as a template for a DNA polymerase reaction, and each containing one of the four dideoxynucleotides

• What is the Polymerase Chain Reaction?

  The solution discusses what is the Polymerase Chain Reaction.

• How to sythesize DNA

  One such method is known as PCR or Polymerase Chain Reaction. This process has revolutionized DNA cloning.

• DNA Replications, Testing, and PCR

  The requirements of the polymerase chain reaction ( PCR) are that there has to be a double stranded DNA template containing the target sequence and two primers that must flank the target DNA template.

• DNA Sequencing Methodology: Dideoxy and Pyrosequencing

  For DNA sequencing using the chain-termination method (also termed Sanger or dideoxy method), four reactions are performed, each using theDNA to be analyzed as a template for a DNA polymerase reaction, and each containing one of the four dideoxynucleotides

• mRNA analysis in the lab

  RT-PCR (reverse transcriptase polymerase chain reaction)-a reverse transcriptase transcribes mRNAs to cDNAs; the cDNA of interest is amplified by PCR -shows the mRNA is made under given conditions, and can be semi-quantitative 6. real time PCR: similar

• Designing primers for PCR: finding problems with primers

  173612 Designing primers for PCR: finding problems with primers 1- Why is the use of temperature-stable DNA polymerase an important factor in the polymerase chain reaction? 2- Each of the following pairs of primers have a problem with it.